6+/-0.42, 2.11+/-0.06 (n=6) and 10.5+/-0.31 and 1.19+/-0.02 (n=9), respectively. Detection limits in extracts from muscle fibre fragments were 2.0, 1.0, 3.0 and 2.0 mmol/kg dm, respectively. The assay was found successful for analysis of fibre-fragments weighing > or = 1 microg.
These techniques were largely developed by Arnold de Haan in the Department of Professor Anthony J Sargeant. The data was collected by their talented Greek PhD student Christina Karatzaferi.
Improved high-performance liquid chromatographic assay for the determination of “high-energy” phosphates in mammalian skeletal muscle. Application to a single-fibre study in man
Journal of Chromotography
J Chromatogr B Biomed Sci Appl. 1999 Jul 9;730(2):183-91